Article
Calpain mutants with increased Ca2+ sensitivity and implications for the role of the C(2)-like domain.
The Journal of biological chemistry - 9 Mar 2001
Hosfield C M, Moldoveanu T, Davies P L, Elce J S, Jia Z
Abstract excerpt
The ubiquitous calpain isoforms (mu- and m-calpain) are Ca(2+)-dependent cysteine proteases that require surprisingly high Ca(2+) concentrations for activation in vitro ( approximately 50 and approximately 300 microm, respectively). The molecular basis of such a high requirement for Ca(2+) in vitro is not known. In this study, we substantially reduced the concentration of Ca(2+) required for the activation of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.