Article
Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II.
Biochemical and biophysical research communications - 29 May 1997
Suzuki Y, Ohkura R, Sugiura S, Yasuda R, Kinoshita K, Tanokura M, Sutoh K
Abstract excerpt
The cysteine residue called SH2 in the skeletal myosin heavy chain is conserved among various species. Cys 678 in Dictyostelium myosin II is equivalent to SH2 in skeletal myosin. Using the Dictyostelium myosin II heavy chain gene, SH2 was mutated to Gly, Ala, Ser, or Thr. These mutant myosins wer...
Topics
- Actins
- Animals
- Ca(2+) Mg(2+)-ATPase
- Cysteine
- Dictyostelium
- Mutagenesis, Site-Directed
- Myosins
- Phenotype
- src Homology Domains