Article
Protein kinase C contains two phorbol ester binding domains.
The Journal of biological chemistry - 25 Sept 1991
Burns D J, Bell R M
Abstract excerpt
A series of deletion and truncation mutants of protein kinase C (PKC) were expressed in the baculovirus-insect cell expression system in order to elucidate the ability of various domains of the enzyme to bind phorbol dibutyrate (PDBu). A PKC truncation mutant consisting of only the catalytic domain of the enzyme did not bind [3H]PDBu, whereas a PKC truncation mutant consisting of the regulatory domain (containing...
Topics
- Amino Acid Sequence
- Baculoviridae
- Base Sequence
- Blotting, Western
- Catalysis
- DNA
- Electrophoresis, Polyacrylamide Gel
- Genes, Viral
- Isoenzymes
- Molecular Sequence Data
- Mutation
- Phorbol 12,13-Dibutyrate