Article
Dynamic re-distribution of protein kinase D (PKD) as revealed by a GFP-PKD fusion protein: dissociation from PKD activation.
FEBS letters - 3 Sept 1999
Matthews S, Iglesias T, Cantrell D, Rozengurt E
Abstract excerpt
Protein kinase D (PKD)/protein kinase Cmicro (PKCmicro, a serine/threonine protein kinase with distinct structural and enzymological properties, is rapidly activated in intact cells via PKC. The amino-terminal region of PKD contains a cysteine-rich domain (CRD) that directly binds phorbol esters with a high affinity. Here, we show that treatment of transfected RBL 2H3 cells with phorbol 12,13-dibutyrate (PDB)...
Topics
- Amino Acid Substitution
- Animals
- Biological Transport
- COS Cells
- Cell Membrane
- Cysteine
- Cytosol
- Enzyme Activation
- Mast Cells
- Mutation
- Phorbol 12,13-Dibutyrate
- Protein Kinase C
- Recombinant Fusion Proteins