Article
Lack of B-RAF mutations in head and neck squamous cell carcinoma.
Folia biologica - 1 Jan 2008
Al Sheikh Ali M, Gunduz M, Gunduz E, Tamamura R, Beder L, Tominaga S, Onoda T, Yamanaka N, Grenman R, Shimizu K, Nagai N, Nagatsuka H
Abstract excerpt
B-RAF is one of the most commonly mutated oncogenes in human cancer. However, the mutation status of B-RAF has not been established completely in HNSCC. We have analysed the mutation status of the kinase domain of the B-RAF gene (exons 11 and 15) in 91 Japanese HNSCC patients as well as 12 HNSCC cell lines. DNA was extracted and amplified by PCR. Mutations were then analysed by SSCP mutation detection method....
Topics
- Alleles
- Carcinoma, Squamous Cell
- Cell Line, Tumor
- DNA Mutational Analysis
- Exons
- Head and Neck Neoplasms
- Humans
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational