Application of a simplified PCR-SSP method to detect A4GALT*01 and A4GALT*02 typing among Thai blood donors.

doi:10.1016/j.tracli.2023.08.007

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Article

Application of a simplified PCR-SSP method to detect A4GALT01 and A4GALT02 typing among Thai blood donors.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine - 1 Nov 2023

Amphonritthisak Pattaranit, Nathalang Oytip, Leetrakool Nipapan, Mitundee Supattra, Intharanut Kamphon

Abstract excerpt

OBJECTIVES: An intronic A4GALT single nucleotide variant, rs5751348:G>T, P2 or A4GALT*02 allele has a lower level of the enzyme-encoding A4GALT transcripts than the P1 individuals. Here, we first develop and validate a simple inhouse PCR-SSP method to detect A4GALT*01 and A4GALT*02 alleles, and second, apply this method to compare the allele frequencies between Thai and other populations. MATERIAL AND METHODS:...

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PMID: 37673392
DOI: 10.1016/j.tracli.2023.08.007

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