Article
Two N-linked glycosylation sites (Asn18 and Asn106) are both required for full enzymatic activity, thermal stability, and resistance to proteolysis in mammalian deoxyribonuclease I.
Bioscience, biotechnology, and biochemistry - 1 Dec 2008
Fujihara Junko, Yasuda Toshihiro, Kunito Takashi, Fujii Yoshimi, Takatsuka Hisakazu, Moritani Tamami, Takeshita Haruo
Abstract excerpt
Deoxyribonuclease I (DNase I) is known to be a glycoprotein, and two potential N-linked glycosylation sites (N18 and N106) are known for mammalian enzymes. In the present study, N18 and N106 were mutated in order to investigate the biological role of N-linked glycosylation in three mammalian (human, bovine, and equine) DNases I. The enzyme activities of N18Q and N106Q were lower than that of the wild type, and...
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