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Article

Probing the folding transition state of ubiquitin mutants by temperature-jump-induced downhill unfolding.

Biochemistry - 30 Dec 2008

Chung Hoi Sung, Shandiz Ali, Sosnick Tobin R, Tokmakoff Andrei

Abstract excerpt

Crucial to revealing mechanistic details of protein folding is a characterization of the transition state ensemble and its structural dynamics. To probe the transition state of ubiquitin thermal unfolding, we examine unfolding dynamics and kinetics of wild-type and mutant ubiquitin using time-resolved nonlinear infrared spectroscopy after a nanosecond temperature jump. We observe spectral changes on two different...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19053229
DOI: 10.1021/bi801603e
PMCID: PMC3319365

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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