Article
Evidence for a three-state model of protein folding from kinetic analysis of ubiquitin variants with altered core residues.
Nature structural biology - 1 Feb 1996
Khorasanizadeh S, Peters I D, Roder H
Abstract excerpt
To elucidate the kinetic importance of structural intermediates in single-domain proteins, we measured the effect of solution conditions and amino-acid changes at a central core residue of ubiquitin (Val 26) on the kinetics of folding and unfolding. Kinetic analysis in terms of a sequential three...
Topics
- Humans
- Kinetics
- Models, Chemical
- Models, Molecular
- Mutation
- Protein Denaturation
- Protein Folding
- Spectrometry, Fluorescence
- Ubiquitins
- Valine