Article
Activation of the trpBA promoter of Pseudomonas aeruginosa by TrpI protein in vitro.
Journal of bacteriology - 1 Jun 1991
Gao J G, Gussin G N
Abstract excerpt
We have developed an in vitro transcription system in which purified TrpI protein and indoleglycerol phosphate (InGP) activate transcription initiation at the trpBA promoter (trpPB) and repress initiation at the trpI promoter (trpPI) of Pseudomonas aeruginosa. The phenotypes resulting from mutations in the -10 region of both promoters indicate that the -10 region consensus sequence in P. aeruginosa is probably...
Topics
- Bacterial Proteins
- Bacteriophages
- Base Sequence
- DNA-Binding Proteins
- Genes, Bacterial
- Genes, Viral
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Promoter Regions, Genetic
