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Article

Site-directed mutagenesis of dicarboxylic acids near the active site of Bacillus cereus 5/B/6 beta-lactamase II.

The Biochemical journal - 1 Jun 1991

Lim H M, Iyer R K, Pène J J

Abstract excerpt

An amino acid residue functioning as a general base has been proposed to assist in the hydrolysis of beta-lactam antibiotics by the zinc-containing Bacillus cereus beta-lactamase II [Bicknell & Waley (1985) Biochemistry 24, 6876-6887]. Oligonucleotide-directed mutagenesis of cloned Bacillus cereu...

Topics

Amino Acid Sequence
Asparagine
Aspartic Acid
Bacillus cereus
Base Sequence
Binding Sites
Blotting, Western
Cephalosporinase
Cloning, Molecular
Escherichia coli
Genetic Vectors
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Phenotype

Recombinant Proteins

Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 1904717
DOI: 10.1042/bj2760401
PMCID: PMC1151105

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1904717-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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