Article
One-step generation of double-allele gene replacement mutants in Leishmania donovani.
International journal for parasitology - 1 Apr 2009
Ommen Gabi, Lorenz Sabine, Clos Joachim
Abstract excerpt
Due to the apparent lack of sexual recombination in Leishmania spp., gene replacement strategies in this diploid organism necessitate the subsequent targeting of two gene alleles by using two constructs, bearing different antibiotic resistance markers. This approach is time consuming and often gives rise to spontaneous amplification of the targeted gene, nullifying efforts to create functional null mutants. Here,...
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