Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi

11 May 2009

Abstract excerpt

BACKGROUND: Trypanosoma cruzi, a kinetoplastid protozoan parasite that causes Chagas disease, infects approximately 15 million people in Central and South America. In contrast to the substantial in silico studies of the T. cruzi genome, transcriptome, and proteome, only a few genes have been experimentally characterized and validated, mainly due to the lack of facile methods for gene manipulation needed for...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biochemical and Molecular Research
Share to Trypanosoma species research and implications
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 19432966
DOI: 10.1186/1471-2180-9-90

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evaluation of high efficiency gene knockout strategies for Trypanosoma cruziPMID 19432966Read ↗

Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture.Semantic proximity Read ↗

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruziSemantic proximity Read ↗

Expression of exogenous genes in Trypanosoma cruzi : improving vectors and electroporation protocolsSemantic proximity Read ↗

A limitation lifted: A conditional knockdown system reveals essential roles for Polo-like kinase and Aurora kinase 1 in Trypanosoma cruzi cell divisionSemantic proximity Read ↗

High throughput screening of <i>Toxoplasma gondii</i> clones by PCR after limitation cloningSemantic proximity Read ↗

Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9Semantic proximity Read ↗

Genetically Validated Drug Targets in Leishmania: Current Knowledge and Future ProspectsSemantic proximity Read ↗

Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivoSemantic proximity Read ↗

Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasiteSemantic proximity Read ↗

Assessment of two CRISPR-Cas9 genome editing protocols for rapid generation of Trypanosoma cruzi gene knockout mutants.Semantic proximity Read ↗

A toolbox for conditional control of gene expression in apicomplexan parasitesSemantic proximity Read ↗

Chromosome-wide analysis of gene function by RNA interference in the african trypanosome.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.