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Article

Leishmania major HEXBP deletion mutants generated by double targeted gene replacement.

Molecular and biochemical parasitology - 1 Feb 1994

Webb J R, McMaster W R

Abstract excerpt

The Leishmania major single-stranded DNA binding protein HEXBP contains nine 'CCHC' zinc finger motifs and binds to oligodeoxynucleotides derived from the antisense strand of the GP63 gene 5' flanking region in gel mobility shift assays and UV-crosslinking assays. In the present study a HEXBP-deficient clone of L. major was generated by double targeted gene replacement. The two HEXBP alleles were sequentially...

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PMID: 8008021
DOI: 10.1016/0166-6851(94)90059-0

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