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Article

Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture.

Memorias do Instituto Oswaldo Cruz - 1 Jul 2014

Alcantara Monica Visnieski, Fragoso Stenio Perdigão, Picchi Gisele Fernanda Assine

Abstract excerpt

Gene knockout is a widely used approach to evaluate loss-of-function phenotypes and it can be facilitated by the incorporation of a DNA cassette having a drug-selectable marker. Confirmation of the correct knockout cassette insertion is an important step in gene removal validation and has general...

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PMID: 24936912
DOI: 10.1590/0074-0276140010
PMCID: PMC4155859

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-24936912-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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