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Article

Selection of yeast strains with enhanced expression of Plasmodium falciparum proteins.

Molecular and biochemical parasitology - 1 Feb 2009

LaCount Douglas J, Schoenfeld Lori W, Fields Stanley

Abstract excerpt

The poor expression of Plasmodium falciparum proteins in heterologous systems and the difficulty in obtaining sufficient material directly from the parasite have limited the experimental characterization of many of the approximately 5200 proteins encoded by this organism. To improve the expression of P. falciparum proteins in the yeast Saccharomyces cerevisiae, we selected yeast ura3 mutants that acquired the...

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Identifiers

PMID: 19026694
DOI: 10.1016/j.molbiopara.2008.10.003
PMCID: PMC2629795

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