Article
Efficient gene replacement and direct hyphal transformation in Sclerotinia sclerotiorum.
Molecular plant pathology - 1 Sept 2008
Levy M, Erental A, Yarden O
Abstract excerpt
Homologous recombination is required for gene-targeted procedures such as gene disruption and gene replacement. Ku80 is part of the non-homologous end-joining DNA repair mechanism in many organisms. We identified and disrupted the Ku80 homologue in Sclerotinia sclerotiorum and generated heterokaryon mutants enriched with Ku80-deficient nuclei (ssku80). Sclerotial formation and pathogenicity of ssku80 mutants were...
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