Article

A Protoplast Transformation System for Gene Deletion and Complementation in Sclerotinia sclerotiorum

2013-12-01

Abstract excerpt

Protoplast transformation is an important technique for establishing a mutation library and determining gene function for Sclerotinia sclerotiorum and other plant pathogenic fungi. In this study, we determined the effect of various conditions on preparation of protoplasts for transformation. These conditions included the age of the culture providing the hyphae to be digested; enzyme composition, buffer solution an...

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Literature Corpus work: 84117f4c-33c9-5695-8341-345ec2a2a03e
DOI: 10.1111/jph.12137

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