Article
Mutagenesis of the human transferrin receptor: two cytoplasmic phenylalanines are required for efficient internalization and a second-site mutation is capable of reverting an internalization-defective phenotype.
The Journal of cell biology - 1 Mar 1991
McGraw T E, Pytowski B, Arzt J, Ferrone C
Abstract excerpt
Site-specific mutagenesis has been used to define the sequences required for efficient internalization of the human transferrin receptor. It has previously been shown that the sole cytoplasmic tyrosine, at position 20, is required for efficient internalization. When two other cytoplasmic aromatic...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- Coated Pits, Cell-Membrane
- Cricetinae
- Cytoplasm
- Endocytosis
- Humans
- Iron
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Phenylalanine
- Receptors, Transferrin