Article
Thrombin cleavage analysis of a novel antihaemophilic factor variant, factor VIII delta II.
European journal of biochemistry - 14 Feb 1991
Krishnan S, Kolbe H V, Lepage P, Faure T, Sauerwald R, de la Salle H, Muller C, Bihoreau N, Paolantonacci P, Roitsch C
Abstract excerpt
Factor VIII delta II is a genetically engineered deletion variant of factor VIII expressed by recombinant Chinese hamster ovary cells, in which a major portion of the central (B) domain and a part of the light chain (Pro771-Asp1666) are missing. After immunoaffinity purification, the kinetics of thrombin cleavage of the novel molecule was analysed by SDS/PAGE, Western blotting and N-terminal amino acid...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Western
- Cell Line
- Chromatography, High Pressure Liquid
- Chromosome Deletion
- Electrophoresis, Polyacrylamide Gel
- Factor VII
- Factor VIII
- Genetic Variation