Article
Biological activity of recombinant factor VIII variants lacking the central B-domain and the heavy-chain sequence Lys713-Arg740: discordant in vitro and in vivo activity.
British journal of haematology - 1 Sept 1993
Mertens K, Donath M J, van Leen R W, de Keyzer-Nellen M J, Verbeet M P, Klaasse Bos J M, Leyte A, van Mourik J A
Abstract excerpt
Recombinant factor VIII variants with overlapping deletions spanning the region Lys713-Ile1668 have been expressed in mammalian cells, and analysed for biological activity both in vitro and in vivo. Two distinct assay systems were used to measure the activity in vitro. The one-stage coagulation a...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blood Coagulation
- Chromosome Deletion
- Dogs
- Electrophoresis, Polyacrylamide Gel
- Factor VIII
- Factor X
- Genetic Variation
- Hemophilia A
- Immunoblotting
- Male
- Mice
- Molecular Sequence Data