Article
Cleavage at Arg-1689 influences heavy chain cleavages during thrombin-catalyzed activation of factor VIII.
The Journal of biological chemistry - 24 Apr 2009
Newell Jennifer L, Fay Philip J
Abstract excerpt
The procofactor, factor VIII, is activated by thrombin or factor Xa-catalyzed cleavage at three P1 residues: Arg-372, Arg-740, and Arg-1689. The catalytic efficiency for thrombin cleavage at Arg-740 is greater than at either Arg-1689 or Arg-372 and influences reaction rates at these sites. Because cleavage at Arg-372 appears rate-limiting and dependent upon initial cleavage at Arg-740, we investigated whether...
Topics
- Arginine
- Catalysis
- Enzyme-Linked Immunosorbent Assay
- Factor VIII
- Factor Xa
- Humans
- Kinetics
- Mutation
- Protein Binding
- Protein Structure, Tertiary
- Recombinant Proteins