Analysis of arginine-rich peptides from the HIV Tat protein reveals unusual features of RNA-protein recognition.

Abstract excerpt

Arginine-rich sequences are found in many RNA-binding proteins and have been proposed to mediate specific RNA recognition. Fragments of the HIV-1 Tat protein that contain the arginine-rich region of Tat bind specifically to a 3-nucleotide bulge in TAR RNA. To determine the amino acid requirements for specific RNA recognition, we synthesized a series of mutant Tat peptides spanning this domain (YGRKKRRQRRRP) and...

Topics

• Amino Acid Sequence
• Arginine
• Base Sequence
• Carrier Proteins
• Circular Dichroism
• Gene Products, tat
• HIV Long Terminal Repeat
• HIV-1
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation