Article
Construction and propagation of deletion derivatives of staphylococcal tetracycline-resistance plasmid pTP-5 in Bacillus subtilis.
Gene - 1 Jan 2000
Noguchi N, Shishido K, Ando T, Kono M
Abstract excerpt
Tetracycline-resistance (TCr) plasmid pTP-5 (4.46 kb) from Staphylococcus aureus was cleaved with HindIII into three fragments: A (2.35 kb), B (1.57 kb) and C (0.54 kb). A deletion plasmid (3.92 kb) lacking HindIII-C fragment was obtained, and was designated pNS1. This plasmid retained the TCr ph...
Topics
- Bacillus subtilis
- Chromosome Deletion
- Chromosomes, Bacterial
- DNA Restriction Enzymes
- Drug Resistance, Microbial
- Phenotype
- Plasmids
- Staphylococcus aureus
- Tetracycline