Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

MethyLight: a high-throughput assay to measure DNA methylation.

Nucleic acids research - 15 Apr 2000

Eads C A, Danenberg K D, Kawakami K, Saltz L B, Blake C, Shibata D, Danenberg P V, Laird P W

Abstract excerpt

Cytosine-5 DNA methylation occurs in the context of CpG dinucleotides in vertebrates. Aberrant methylation of CpG islands in human tumors has been shown to cause transcriptional silencing of tumor-suppressor genes. Most methods used to analyze cytosine-5 methylation patterns require cumbersome manual techniques that employ gel electrophoresis, restriction enzyme digestion, radiolabeled dNTPs or hybridization...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adaptor Proteins, Signal Transducing
Share to Adenocarcinoma

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CpG Islands

Share to DNA Repair

Share to MutL Protein Homolog 1

Share to Neoplasm Proteins

Share to Oligonucleotide Probes

Share to Sulfites

Source

Open in PubMed Open full text

Identifiers

PMID: 10734209
DOI: 10.1093/nar/28.8.e32
PMCID: PMC102836

Genes

MLH1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

MethyLight: a high-throughput assay to measure DNA methylation.PMID 10734209Read ↗

Genome-scale DNA methylation analysis.Semantic proximity Read ↗

Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancerSemantic proximity Read ↗

MethylQuant: a real-time PCR-based method to quantify DNA methylation at single specific cytosines.Semantic proximity Read ↗

A DNA alteration and methylation co-detection method for clinical purpose.Semantic proximity Read ↗

CpG island methylator phenotype underlies sporadic microsatellite instability and is tightly associated with BRAF mutation in colorectal cancer.Semantic proximity Read ↗

IMPRESS: Improved methylation profiling using restriction enzymes and smMIP sequencing, combined with a new biomarker panel, creating a multi-cancer detection assaySemantic proximity Read ↗

The DNA methylome: a novel biomarker.Semantic proximity Read ↗

An Integrative DNA Sequencing and Methylation Panel to Assess Mismatch Repair Deficiency.Semantic proximity Read ↗

Methyl-CODEC enables simultaneous methylation and duplex sequencingSemantic proximity Read ↗

Genome-wide analysis of DNA methylation patterns.Semantic proximity Read ↗

Quantitative DNA methylation analysis: the promise of high-throughput epigenomic diagnostic testing in human neoplastic disease.Semantic proximity Read ↗

Enhanced Performance of DNA Methylation Markers by Simultaneous Measurement of Sense and Antisense DNA Strands after Cytosine ConversionSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.