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Enumeration of Mycobacterium leprae Using Real-Time PCR

4 Nov 2008

Abstract excerpt

Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae DNA in biological samples. Primers were identified to amplify a shared region of the multicopy repeat sequence (RLEP) specific to M. leprae and tested for...

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PMID: 18982056
DOI: 10.1371/journal.pntd.0000328

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