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Rapid and sensitive detection of Mycobacterium leprae using a nested-primer gene amplification assay

1 Sept 1990

Abstract excerpt

By using a set of four nested oligonucleotide primers, a two-step polymerase chain reaction assay for the detection and identification of Mycobacterium leprae that does not require the use of radioactivity labeled hybridization probes was developed. The nested-primer procedure amplified a 347-base-pair product from M. leprae genomic DNA. No amplification products were produced from DNAs of 19 other Mycobacterium...

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PMID: 2229372
DOI: 10.1128/jcm.28.9.1913-1917.1990

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