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Counting Mycobacteria in Infected Human Cells and Mouse Tissue: A Comparison between qPCR and CFU

19 Apr 2012

Abstract excerpt

Due to the slow growth rate and pathogenicity of mycobacteria, enumeration by traditional reference methods like colony counting is notoriously time-consuming, inconvenient and biohazardous. Thus, novel methods that rapidly and reliably quantify mycobacteria are warranted in experimental models to facilitate basic research, development of vaccines and anti-mycobacterial drugs. In this study we have developed...

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PMID: 22532835
DOI: 10.1371/journal.pone.0034931

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