Article
A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development.
The Journal of experimental medicine - 24 Nov 2008
Jani Anant, Wan Mimi, Zhang Jianmin, Cui Kairong, Wu Jie, Preston-Hurlburt Paula, Khatri Rohini, Zhao Keji, Chi Tian
Abstract excerpt
We have developed a general strategy for creating littermates bearing either a tissue-specific point mutation or deletion in any target gene, and used the method to dissect the roles of Brg, the ATPase subunit of the chromatin-remodeling Brg-associated factor (BAF) complex, in early thymocyte development. We found that a point mutation that inactivates the Brg ATPase recapitulates multiple defects previously...
Topics
- Animals
- CD4 Antigens
- Chromatin
- Chromosomal Proteins, Non-Histone
- DNA Helicases
- Gene Deletion
- Gene Expression Regulation
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic