Article
Novel human PDGFA gene transcripts derived by alternative mRNA splicing.
Gene - 15 Feb 1991
Sánchez A, Chesterman C N, Sleigh M J
Abstract excerpt
The polymerase chain reaction (PCR) has been used to amplify sequences coding for the platelet-derived growth factor A chain (PDGFA) using mRNA populations derived from two transformed cell lines (a human osteosarcoma, U-2OS, and a human glioma, U-343) and from human umbilical vein cells. The pri...
Topics
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA
- Exons
- Genetic Variation
- Glioma
- Humans
- Macromolecular Substances
- Molecular Sequence Data
- Oligonucleotide Probes
- Osteosarcoma
- Platelet-Derived Growth Factor
- Polymerase Chain Reaction
- RNA Splicing
- RNA, Messenger
- Transcription, Genetic
- Umbilical Veins