Article
Quantitation of low abundance mRNAs in glial cells using different polymerase chain reaction (PCR)-based methods.
Brain research. Brain research protocols - 1 Aug 1997
Santagati S, Garnier M, Carlo P, Violani E, Picotti G B, Maggi A
Abstract excerpt
The conventional methods for mRNA quantitation such as Northern blotting or ribonuclease protection assay sometimes lack enough sensitivity to study low abundance mRNAs or to work with limited amounts of biological samples. The sensitivity of the polymerase chain reaction (PCR) linked to reverse transcription (RT-PCR) has proven useful in amplifying specific mRNAs, especially those present in low copy number....
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