Article
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
Genes & development - 1 Sept 1991
Bond U M, Yario T A, Steitz J A
Abstract excerpt
To study the role of base-pairing between the mammalian U7 snRNA and the highly variable histone downstream element (HDE) during the 3'-end maturation of mammalian histone pre-mRNAs, we mutated the HDE of the mouse H2A-614 gene and assayed processing in HeLa cells both in vivo and in vitro. Eithe...
Topics
- Animals
- Base Composition
- Base Sequence
- Gene Expression
- Genes, Synthetic
- HeLa Cells
- Histones
- Humans
- Mice
- Molecular Sequence Data
- Mutation
- RNA Precursors
- RNA Processing, Post-Transcriptional
- RNA, Small Nuclear
- Suppression, Genetic
- Transfection