Article
Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable.
Molecular & general genetics : MGG - 1 Jul 1997
Muth G, Frese D, Kleber A, Wohlleben W
Abstract excerpt
Temperature-sensitive integration plasmids carrying internal fragments of the Streptomyces lividans TK24 recA gene were constructed and used to inactivate the chromosomal recA gene of S. lividans by gene disruption and gene replacement. Integration of these plasmids resulted in recA mutants expressing C-terminally truncated RecA proteins, as deduced from Southern hybridization experiments. Mutants FRECD2 in which...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
