Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable.

doi:10.1007/s004380050514

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Article

Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable.

Molecular & general genetics : MGG - 1 Jul 1997

Muth G, Frese D, Kleber A, Wohlleben W

Abstract excerpt

Temperature-sensitive integration plasmids carrying internal fragments of the Streptomyces lividans TK24 recA gene were constructed and used to inactivate the chromosomal recA gene of S. lividans by gene disruption and gene replacement. Integration of these plasmids resulted in recA mutants expre...

Topics

Gene Expression Regulation, Bacterial
Genes, Bacterial
Genes, Lethal
Mutagenesis, Insertional
Mutation
Phenotype
Rec A Recombinases
Sequence Deletion
Species Specificity
Streptomyces

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