Article
[Partial suppression of rec B rec C mutations in E. coli by plasmid pBR 322 containing a Bacillus subtilis chromosome insertion].
TSitologiia i genetika - 1 Jan 2000
Barabanshchikov B I, Bashkirov V I, Gizatullin F Sh, Gulitashvili V Sh
Abstract excerpt
ATP-dependent DNAase genes of Bac. subtilis were originally cloned in E. coli plasmid pBR322. These genes are expressed in rec BC mutant E. coli cells leading to a complete recovery of the enzyme activity. Bac. subtilis enzyme suppresses reparative properties of the rec BC mutant to a considerabl...
Topics
- Bacillus subtilis
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA Transposable Elements
- DNA, Bacterial
- Deoxyribonuclease BamHI
- Escherichia coli
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Mutation
- Plasmids
- Recombination, Genetic
- Suppression, Genetic