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Removal of high-molecular-weight DNA by carboxylated magnetic beads enhances the detection of mutated K-ras DNA in urine.

Annals of the New York Academy of Sciences - 1 Aug 2008

Su Ying-Hsiu, Song Janet, Wang Zhili, Wang Xiao-He, Wang Mengjun, Brenner Dean E, Block Timothy M

Abstract excerpt

We have previously demonstrated that mutated DNA derived from the circulation can be detected in urine and predominantly exists as DNA fragments <1 kb. To preferentially isolate the trans-renal DNA from urine, we developed a method using carboxylated beads to separate high-MW (1 kb or larger) from low-MW DNA in urine. A primer set for 18s rRNA (generating a PCR product of 872 bp) was designed and optimized for...

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Identifiers

PMID: 18837929
DOI: 10.1196/annals.1448.019
PMCID: PMC2572261

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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