Article
Next-Generation Sequencing-Based Analysis of Urine Cell-Free mtDNA Reveals Aberrant Fragmentation and Mutation Profile in Cancer Patients.
Clinical chemistry - 31 Mar 2022
Zhou Kaixiang, Liu Yang, Yuan Qing, Lai Dong, Guo Shanshan, Wang Zhenni, Su Liping, Zhang Huanqin, Wang Xiangxu, Guo Wenjie, Ji Xiaoying, Gu Xiwen, Huang Qichao, Guo Xu, Xing Jinliang
Abstract excerpt
BACKGROUND: Many studies have demonstrated the high efficacy of cell-free nuclear DNA in cancer diagnostics. Compared to nuclear DNA, mitochondrial DNA (mtDNA) exhibits distinct characteristics, including multiple copies per cell and higher mutation frequency. However, the potential applicability of cell-free mtDNA (cf-mtDNA) in plasma and urine remains poorly investigated. METHODS: Here, we comprehensively...
Topics
- Animals
- DNA, Mitochondrial
- High-Throughput Nucleotide Sequencing
- Humans
- Leukocytes, Mononuclear
- Mutation
- Neoplasms