Article
Single nucleotide polymorphism (SNP) in prion protein gene (PRNP) exon-3 in Gaddi sheep.
Developments in biologicals - 1 Jan 2008
Gupta N, Choudhary S, Malik G, Pandey A, Gupta S C
Abstract excerpt
Nine PCR-SSCP conformations in PRNPthe exon-3 genomic DNA segment covering codons 136, 154 and 171 were observed. DNA sequences revealed 9 unique variants (DQ885789-DQ885796). At codon 171 of exon-3, two alleles, Q and R, had a frequency of 0.336 and 0.664, respectively. Genotypes QQ, QR and RR were observed in 25.86, 15.51 and 58.62% of animals, respectively, showing deviation from Hardy-Weinberg equilibrium. At...
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