Article
Mutation scanning and genotyping by high-resolution DNA melting analysis in olive germplasm.
Genome - 1 Mar 2009
Muleo Rosario, Colao Maria Chiara, Miano Dario, Cirilli Marco, Intrieri Maria C, Baldoni Luciana, Rugini Eddo
Abstract excerpt
The application of high-resolution melting (HRM) analysis of DNA is reported for scanning and genotyping Olea europaea germplasm. To test the sensitivity of the method, a functional gene marker, phytochrome A (phyA), was used, since this gene is correlated with important traits for the ecology of the species. We have designed a set of oligos able to produce amplicons of 307 bp to scan for the presence of single...
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