Article
In-gel multiple displacement amplification of long DNA fragments diluted to the single molecule level.
Analytical biochemistry - 15 Dec 2008
Michikawa Yuichi, Sugahara Keisuke, Suga Tomo, Ohtsuka Yoshimi, Ishikawa Kenichi, Ishikawa Atsuko, Shiomi Naoko, Shiomi Tadahiro, Iwakawa Mayumi, Imai Takashi
Abstract excerpt
The isolation and multiple genotyping of long individual DNA fragments are needed to obtain haplotype information for diploid organisms. Limiting dilution of sample DNA followed by multiple displacement amplification is a useful technique but is restricted to short (<5 kb) DNA fragments. In the current study, a novel modification was applied to overcome these problems. A limited amount of cellular DNA was...
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