Article
Characterization of putative hydrophobic substrate binding site residues of a Delta class glutathione transferase from Anopheles dirus.
Archives of biochemistry and biophysics - 1 Nov 2008
Lerksuthirat Tassanee, Ketterman Albert J
Abstract excerpt
To date, investigations of the hydrophobic substrate site of the insect Delta class glutathione transferase are limited in number. In the present study, putative hydrophobic site residues of AdGSTD4-4 have been proposed and characterized. These residues are Gln-112, Thr-174, Phe-212, Arg-214, Tyr...
Topics
- Animals
- Anopheles
- Binding Sites
- Catalysis
- Enzyme Stability
- Escherichia coli
- Glutathione Transferase
- Hot Temperature
- Hydrogen Bonding
- Hydrophobic and Hydrophilic Interactions
- Kinetics
- Models, Chemical
- Models, Molecular
- Mutation
- Protein Binding
- Protein Engineering
- Recombinant Proteins
- Substrate Specificity