Article
The catalytic site of glutathione peroxidases.
Antioxidants & redox signaling - 1 Sept 2008
Tosatto Silvio C E, Bosello Valentina, Fogolari Federico, Mauri Pierluigi, Roveri Antonella, Toppo Stefano, Flohé Leopold, Ursini Fulvio, Maiorino Matilde
Abstract excerpt
In GPxs, the redox-active Se or S, is at hydrogen bonding distance from Gln and Trp residues that contribute to catalysis. From sequence homology of >400 sequences and modeling of the DmGPx as a paradigm, Asn136 emerged as a fourth essential component of the active site. Mutational substitution of Asn136 by His, Ala, or Asp results in a dramatic decline of specific activity. Kinetic analysis indicates that k(+1),...
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