Article
Development and precise characterization of phospho-site-specific antibody of Ser(357) of IRS-1: elimination of cross reactivity with adjacent Ser(358).
Biochemical and biophysical research communications - 7 Nov 2008
Waraich Rizwana Sanaullah, Zaidi Nousheen, Moeschel Klaus, Beck Alexander, Weigert Cora, Voelter Wolfgang, Kalbacher Hubert, Lehmann Rainer
Abstract excerpt
Antibodies that recognize specifically phosphorylated sites on proteins are widely utilized for studying the regulation and biological function of phosphoproteins. The proposed strategy is a powerful, analytical tool allowing the generation of phospho-site specific antibodies albeit adjacent phosphorylation sites are present. Here, we demonstrate the assessment and elimination of cross reactivity of...
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