Article
A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain.
The Journal of biological chemistry - 21 Jun 2002
Kane Susan, Sano Hiroyuki, Liu Simon C H, Asara John M, Lane William S, Garner Charles C, Lienhard Gustav E
Abstract excerpt
This study describes a method for the identification of the substrates of specific serine kinases. An antibody specific for the phosphomotif generated by the kinase is used to isolate phosphorylated substrates by immunoprecipitation, and the isolated proteins are identified by tandem mass spectrometry of peptides. This method was applied to the identification of substrates for the protein kinase Akt, which...
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