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[Construction of intrakine mutant SDF-1alpha/54/KDEL and its inhibiting effects upon CXCR4 expression on cell membrane].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi - 1 Jun 2008

Chen Hongyuan, Tan Yi, Guo Zhigang, Ma Weifeng, Cai Shaoxi, Du Jun, Huang Jun, Hu Houwen, Cai Shaohui

Abstract excerpt

To investigate the impact of phenotypic knockout of CXCR4 on Molt-4 cells via intrakine technology,the C-terminal alpha-helix gene SDF-1alpha/54/KDEL of human stromal cell-derived Faceor-1 deletion is fused to a retention signal 4-peptide -KDEL that retains the newly synthesized receptor within the Molt-4 cells endoplasimc reticulum. Subsequently, PCR is used to amplify the target gene SDF-1alpha/54/ KDEL from...

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Share to Stromal Cells
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 18693449

Genes

CXCR4

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