Article
Characterization and expression of RAD4 gene involved in nucleotide excision repair of UV-damaged Saccharomyces cerevisiae.
The Journal of toxicological sciences - 1 Feb 1991
Choi I S, Park S D
Abstract excerpt
Saccharomyces cerevisiae express RAD4 gene for nucleotide excision repair of UV-induced DNA damages. Upon complementation with rad4-4 mutant, a 7.6 kb clone containing the RAD4 gene designated as pPC1 was isolated from a yeast genomic library. The pPC1 was further narrowed to 2.5 kb flanked with BglII and BamHI sites. The cloned RAD4 gene was found to propagate in E. coli without loss of its complementing...
Topics
- Cloning, Molecular
- DNA Damage
- DNA Repair
- Escherichia coli
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Nucleic Acid Hybridization
- Nucleotides
- Phenotype
- Plasmids
- Restriction Mapping
