Article
Application of a YHB1-GFP reporter to detect nitrosative stress in yeast.
Redox report : communications in free radical research - 1 Jan 2008
Lewinska Anna, Grzelak Agnieszka, Bartosz Grzegorz
Abstract excerpt
Yeast flavohemoglobin (Yhb1p) plays a pivotal role in NO(*) detoxification and has also been implicated in oxidative/reductive stress responses. In this study, we have used a YHB1-GFP reporter expressing a full-length chromosome-tagged Yhb1-GFP fusion protein to monitor changes in flavohemoglobin levels after cell treatment with oxidants, antioxidants and nitric oxide donors. Only nitric oxide donors were found...
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