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Article

2-tier bacterial and in vitro selection of active and methotrexate-resistant variants of human dihydrofolate reductase.

Journal of biomolecular screening - 1 Jul 2008

Fossati Elena, Volpato Jordan P, Poulin Lucie, Guerrero Vanessa, Dugas David-Antoine, Pelletier Joelle N

Abstract excerpt

We report a rapid and reliable 2-tier selection and screen for detection of activity as well as drug-resistance in mutated variants of a clinically-relevant drug-target enzyme. Human dihydrofolate reductase point-mutant libraries were subjected to a 1st-tier bacterial complementation assay, such that bacterial propagation served as an indicator of enzyme activity. Alternatively, when selection was performed in...

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PMID: 18566481
DOI: 10.1177/1087057108318783

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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