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Article

Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads.

FEBS letters - 6 Mar 2002

Takahashi Fumio, Ebihara Takashi, Mie Masayasu, Yanagida Yasuko, Endo Yaeta, Kobatake Eiry, Aizawa Masuo

Abstract excerpt

Ribosome display was applied to the selection of an enzyme. As a model, we selected and amplified the dihydrofolate reductase (DHFR) gene by ribosome display utilizing a wheat germ cell-free protein synthesis system based on binding affinity to its substrate analog, methotrexate, immobilized on a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 11904191
DOI: 10.1016/s0014-5793(02)02334-7

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-11904191-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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