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Deep mutational scanning of <i>Pneumocystis jirovecii</i> dihydrofolate reductase reveals allosteric mechanism of resistance to an antifolate

2023-09-27

Abstract excerpt

Pneumocystis jirovecii is a fungal pathogen that causes pneumocystis pneumonia, a disease that mainly affects immunocompromised individuals. This fungus has historically been hard to study because of our inability to grow it in vitro . One of the main drug targets in P. jirovecii is its dihydrofolate reductase (PjDHFR). Here, by using functional complementation of the baker’s yeast ortholog, we show that PjDHFR...

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Identifiers and source

Literature Corpus work
c5a53ae0-5bef-5708-b6e8-99ab077af553
DOI
10.1101/2023.09.27.559786
Open publication

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Deep mutational scanning of <i>Pneumocystis jirovecii</i> dihydrofolate reductase reveals allosteric mechanism of resistance to an antifolateDOI 10.1101/2023.09.27.559786
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