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Article

The selective detection of mitochondrial superoxide by live cell imaging.

Nature protocols - 1 Jan 2008

Robinson Kristine M, Janes Michael S, Beckman Joseph S

Abstract excerpt

A general protocol is described to improve the specificity for imaging superoxide formation in live cells via fluorescence microscopy with either hydroethidine (HE) or its mitochondrially targeted derivative Mito-HE (MitoSOX Red). Two different excitation wavelengths are used to distinguish the superoxide-dependent hydroxylation of Mito-HE (385-405 nm) from the nonspecific formation of ethidium (480-520 nm)....

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Identifiers

PMID: 18536642
DOI: 10.1038/nprot.2008.56

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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